Peptide Planning, Design, and Manufacturing
Peptide are short proteins composed of amino acid, with diverse biological activities that play important roles in nature. Peptides create a new class of research and therapeutic materials with a variety of applications.
Many naturally occurring peptides function as hormones playing a role in activating or regulating various cellular pathways. In addition to regulatory activity, a number of peptide toxins that interrupt regulatory pathways were identified. Another class of peptide reagents is useful for signal transduction research. Numerous peptide protease substrate and inhibitors are commercially available both for research and therapeutic use. At the last decade the FDA has approved several therapeutic peptides for HIV and cancer therapies. Peptides are also used for some cosmetic applications such as anti-ageing.
Custom peptide synthesis
Peptide Site-Specific Modifications
N-terminal extensions: Biotinylation (addition of biotin), acetylation (addition of acetyl), acylation (addition of fatty acids), etc.
C-terminal variations: standard peptide acid, peptide amide, peptide hydrazide, peptide methyl ester, and peptide alcohol are optional.
Internal modifications: Phosphorylation on Serine, Threonine, or Tyrosine, Glycosylation on Asparagine.
We can also generate peptides with non-conventional modifications for the needs of the customer.
Chromogenic and Fluorogenic Peptides
Peptides can be marked with chromogenic or fluorescent dyes such as: Dinitrophenol, Dansyl, Dabsyl, Fluorescein, and Rhodamine for fluorescent microscopy and ELISA monitoring of the peptide substrates.
Peptides and proteins can be labeled with a wide variety of chromogenic and fluorescent dyes for the entire scaling range of visible light and also markers in the ranges adapted to dedicated microscopy.
Conjugation of peptides to a carrier protein
Conjugation of peptides to a protein carrier for vaccination in animals – the peptide is attached to a protein carrier such as BSA or KLH to induce a stronger immune response.
Alternatively, the peptide can be synthesized or attached to MAPS
MAPS – Multiple Antigen Peptide System – the system based on an inert and branched core molecule to which several peptides are attached, usually 4-8 peptides with a high molar ratio of peptide antigen to the core molecule that can trigger a strong immune response. The MAPS can be produced with the same immunogenic peptide or connect several different peptides representing different sites in the protein.
Combinatorial Libraries
We use portioning-mixing synthesis methodologies to generate “one bead – one compound” libraries on solid support or soluble library mixtures for use in varies applications.
– Random peptide libraries – for screening of peptide ligands
– Epitop specific libraries – specially designed for screening of enzyme substrates and inhibitors.
Both natural and synthetic building blocks can be utilized during library design and synthesis
Peptides for immunologic applications
Conjugation of peptides to protein carriers for immunization – the peptide is attached to protein carrier such as BSA or KLH to induce a stronger immune response.
MAPS – Multiple Antigen Peptide System – The system based on a small inert and branched core molecule onto which a number of peptides are attached – with high molar ratio of peptide antigen to core molecule that can evoke strong immune response.
Using bioinformatic tools to design selected immunogenic peptides from the target protein.
Production of immunogenic peptides by solid-phase Fmoc/Boc synthesis methods. The peptides undergo sample tests to monitor the synthesis during the production steps. At the end of production, the immunogenic peptides are purified and tested by liquid chromatography (HPLC) and mass spectrometry (MS).
We use optimal synthesis methods, and each synthesis step is monitored in order to achieve high yields and high purity levels of the peptides for obtaining specific antibodies later.
The peptides can be synthesized in quantities ranging from 1 gram to 1 kg.
Peptide affinity columns
Affinity columns bound with peptide of choice on variety of matrixes for affinity purification of antibodies, enzymes and receptors.
Antibody production and purification
Design and synthesis of highly immunogenic hapten peptides for a animal immunization, MAPS – Multiple Antigen Peptide Systems, Conjugation to protein carriers, production of peptide specific polyclonal Antibodies, Antibody Affinity purification.
Analytical services for peptide
Analysis of peptides with Reverse Phase HPLC, Amino Acid Analysis, Peptide Sequencing and Mass Spectrometry.